Determination of salicinoids by micro-high-performance liquid chromatography and photodiode array detection.
Identifieur interne : 001F35 ( Main/Exploration ); précédent : 001F34; suivant : 001F36Determination of salicinoids by micro-high-performance liquid chromatography and photodiode array detection.
Auteurs : K F Rubert-Nason ; C J Hedman ; L M Holeski ; R L LindrothSource :
- Phytochemical analysis : PCA [ 1099-1565 ]
Descripteurs français
- KwdFr :
- Alcools benzyliques (composition chimique), Alcools benzyliques (isolement et purification), Calibrage (MeSH), Chromatographie en phase liquide à haute performance (méthodes), Contrôle de qualité (MeSH), Extraits de plantes (composition chimique), Extraits de plantes (isolement et purification), Feuilles de plante (composition chimique), Glucosides (analyse), Glucosides (composition chimique), Glucosides (isolement et purification), Populus (composition chimique), Reproductibilité des résultats (MeSH).
- MESH :
- analyse : Glucosides.
- composition chimique : Alcools benzyliques, Extraits de plantes, Feuilles de plante, Glucosides, Populus.
- isolement et purification : Alcools benzyliques, Extraits de plantes, Glucosides.
- méthodes : Chromatographie en phase liquide à haute performance.
- Calibrage, Contrôle de qualité, Reproductibilité des résultats.
English descriptors
- KwdEn :
- Benzyl Alcohols (chemistry), Benzyl Alcohols (isolation & purification), Calibration (MeSH), Chromatography, High Pressure Liquid (methods), Glucosides (analysis), Glucosides (chemistry), Glucosides (isolation & purification), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Plant Leaves (chemistry), Populus (chemistry), Quality Control (MeSH), Reproducibility of Results (MeSH).
- MESH :
- chemical , analysis : Glucosides.
- chemical , chemistry : Benzyl Alcohols, Glucosides, Plant Extracts.
- chemical , isolation & purification : Benzyl Alcohols, Glucosides, Plant Extracts.
- chemistry : Plant Leaves, Populus.
- methods : Chromatography, High Pressure Liquid.
- Calibration, Quality Control, Reproducibility of Results.
Abstract
INTRODUCTION
Plant-derived salicinoids (conjugates of glucose and salicylate phenolic moieties) are potent modulators of plant-herbivore interactions. We demonstrate the use of micro-high-performance liquid chromatography (μHPLC) with photodiode-array detection (DAD) for quantification of four salicinoids (salicin, salicortin, hydroxycyclohexen-on-oyl salicortin and tremulacin) in methanolic extracts of Populus.
OBJECTIVE
To develop and implement a solvent-conserving μHPLC method to quantify salicinoids in methanolic extracts of Populus tissue.
METHODS
Salicinoids were extracted from Populus tissue into methanol, filtered, and introduced to μHPLC. Extracted analytes were separated on a Zorbax SB C18-column with a binary gradient of methanol and water (with 2% tetrahydrofuran; 20 μL/min), and quantified by DAD (274 nm). We confirmed measurement reliability through standard addition, comparison with an accepted method, and assessment of chromatographic peak purity by ultraviolet absorbance spectra.
RESULTS
Method detection and quantification limits for the salicinoids as a percentage of dry leaf weight were as follows: salicin (0.1%, 0.2%), salicortin (0.001%, 0.02%), hydroxycyclohexen-on-oyl salicortin (0.02%, 0.06%) and tremulacin (0.0006%, 0.002%). Calibrations by external standardisation were linear over 1.5 orders of magnitude with acceptable accuracy and reproducibility.
CONCLUSION
Micro-HPLC can serve as a solvent-conserving alternative to conventional HPLC for quantification of salicinoids in Populus tissue
DOI: 10.1002/pca.2485
PubMed: 24847528
Affiliations:
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uniqKey="Lindroth R" first="R L" last="Lindroth">R L Lindroth</name></author></analytic><series><title level="j">Phytochemical analysis : PCA</title><idno type="eISSN">1099-1565</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Benzyl Alcohols (chemistry)</term><term>Benzyl Alcohols (isolation & purification)</term><term>Calibration (MeSH)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Glucosides (analysis)</term><term>Glucosides (chemistry)</term><term>Glucosides (isolation & purification)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (isolation & purification)</term><term>Plant Leaves (chemistry)</term><term>Populus (chemistry)</term><term>Quality Control (MeSH)</term><term>Reproducibility of Results (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alcools benzyliques (composition chimique)</term><term>Alcools benzyliques (isolement et purification)</term><term>Calibrage (MeSH)</term><term>Chromatographie en phase liquide à haute performance (méthodes)</term><term>Contrôle de qualité (MeSH)</term><term>Extraits de plantes (composition chimique)</term><term>Extraits de plantes (isolement et purification)</term><term>Feuilles de plante (composition chimique)</term><term>Glucosides (analyse)</term><term>Glucosides (composition chimique)</term><term>Glucosides (isolement et purification)</term><term>Populus (composition chimique)</term><term>Reproductibilité des résultats (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucosides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzyl Alcohols</term><term>Glucosides</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Benzyl Alcohols</term><term>Glucosides</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucosides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alcools benzyliques</term><term>Extraits de plantes</term><term>Feuilles de plante</term><term>Glucosides</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Alcools benzyliques</term><term>Extraits de plantes</term><term>Glucosides</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Calibration</term><term>Quality Control</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Calibrage</term><term>Contrôle de qualité</term><term>Reproductibilité des résultats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>INTRODUCTION</b></p><p>Plant-derived salicinoids (conjugates of glucose and salicylate phenolic moieties) are potent modulators of plant-herbivore interactions. We demonstrate the use of micro-high-performance liquid chromatography (μHPLC) with photodiode-array detection (DAD) for quantification of four salicinoids (salicin, salicortin, hydroxycyclohexen-on-oyl salicortin and tremulacin) in methanolic extracts of Populus.</p></div><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>To develop and implement a solvent-conserving μHPLC method to quantify salicinoids in methanolic extracts of Populus tissue.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Salicinoids were extracted from Populus tissue into methanol, filtered, and introduced to μHPLC. Extracted analytes were separated on a Zorbax SB C18-column with a binary gradient of methanol and water (with 2% tetrahydrofuran; 20 μL/min), and quantified by DAD (274 nm). We confirmed measurement reliability through standard addition, comparison with an accepted method, and assessment of chromatographic peak purity by ultraviolet absorbance spectra.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Method detection and quantification limits for the salicinoids as a percentage of dry leaf weight were as follows: salicin (0.1%, 0.2%), salicortin (0.001%, 0.02%), hydroxycyclohexen-on-oyl salicortin (0.02%, 0.06%) and tremulacin (0.0006%, 0.002%). Calibrations by external standardisation were linear over 1.5 orders of magnitude with acceptable accuracy and reproducibility.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>Micro-HPLC can serve as a solvent-conserving alternative to conventional HPLC for quantification of salicinoids in Populus tissue</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24847528</PMID><DateCompleted><Year>2014</Year><Month>12</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1099-1565</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>3</Issue><PubDate><MedlineDate>2014 May-Jun</MedlineDate></PubDate></JournalIssue><Title>Phytochemical analysis : PCA</Title><ISOAbbreviation>Phytochem Anal</ISOAbbreviation></Journal><ArticleTitle>Determination of salicinoids by micro-high-performance liquid chromatography and photodiode array detection.</ArticleTitle><Pagination><MedlinePgn>185-91</MedlinePgn></Pagination><Abstract><AbstractText Label="INTRODUCTION" NlmCategory="BACKGROUND">Plant-derived salicinoids (conjugates of glucose and salicylate phenolic moieties) are potent modulators of plant-herbivore interactions. We demonstrate the use of micro-high-performance liquid chromatography (μHPLC) with photodiode-array detection (DAD) for quantification of four salicinoids (salicin, salicortin, hydroxycyclohexen-on-oyl salicortin and tremulacin) in methanolic extracts of Populus.</AbstractText><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop and implement a solvent-conserving μHPLC method to quantify salicinoids in methanolic extracts of Populus tissue.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Salicinoids were extracted from Populus tissue into methanol, filtered, and introduced to μHPLC. Extracted analytes were separated on a Zorbax SB C18-column with a binary gradient of methanol and water (with 2% tetrahydrofuran; 20 μL/min), and quantified by DAD (274 nm). We confirmed measurement reliability through standard addition, comparison with an accepted method, and assessment of chromatographic peak purity by ultraviolet absorbance spectra.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Method detection and quantification limits for the salicinoids as a percentage of dry leaf weight were as follows: salicin (0.1%, 0.2%), salicortin (0.001%, 0.02%), hydroxycyclohexen-on-oyl salicortin (0.02%, 0.06%) and tremulacin (0.0006%, 0.002%). Calibrations by external standardisation were linear over 1.5 orders of magnitude with acceptable accuracy and reproducibility.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Micro-HPLC can serve as a solvent-conserving alternative to conventional HPLC for quantification of salicinoids in Populus tissue</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rubert-Nason</LastName><ForeName>K F</ForeName><Initials>KF</Initials></Author><Author ValidYN="Y"><LastName>Hedman</LastName><ForeName>C J</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Holeski</LastName><ForeName>L M</ForeName><Initials>LM</Initials></Author><Author ValidYN="Y"><LastName>Lindroth</LastName><ForeName>R L</ForeName><Initials>RL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D023361">Validation Study</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochem Anal</MedlineTA><NlmUniqueID>9200492</NlmUniqueID><ISSNLinking>0958-0344</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001592">Benzyl Alcohols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005960">Glucosides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>29836-40-6</RegistryNumber><NameOfSubstance UI="C095421">tremulacin</NameOfSubstance></Chemical><Chemical><RegistryNumber>4649620TBZ</RegistryNumber><NameOfSubstance UI="C005696">salicin</NameOfSubstance></Chemical><Chemical><RegistryNumber>YI29948E0Q</RegistryNumber><NameOfSubstance UI="C113068">salicortin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001592" MajorTopicYN="N">Benzyl Alcohols</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002138" MajorTopicYN="N">Calibration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005960" MajorTopicYN="N">Glucosides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011786" MajorTopicYN="N">Quality Control</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>5</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>5</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24847528</ArticleId><ArticleId IdType="doi">10.1002/pca.2485</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Hedman, C J" sort="Hedman, C J" uniqKey="Hedman C" first="C J" last="Hedman">C J Hedman</name><name sortKey="Holeski, L M" sort="Holeski, L M" uniqKey="Holeski L" first="L M" last="Holeski">L M Holeski</name><name sortKey="Lindroth, R L" sort="Lindroth, R L" uniqKey="Lindroth R" first="R L" last="Lindroth">R L Lindroth</name><name sortKey="Rubert Nason, K F" 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